|
KMID : 0377219940190010001
|
|
Medical Journal of Chosun Univercity
1994 Volume.19 No. 1 p.1 ~ p.13
|
|
|
Modulation of Preproenkephalin Gene Expression by Reserpine in PC12 Cells
|
|
Park Seok-Ho
Lim Dong-Yoon
Choi Chul-Hee
Kim Sang-Hoon
Park Sang-Hag
Kim Hack-Ryul
|
|
|
Abstract
|
|
|
In cultured PCl2 celIs, prepronkephalin gene expression was studied by measuring preproenkephalin mRNA levels as well as enkephalin content after the treatment of reserpine. Reserpine(0.5 uM) elicited and increased enkephalin peptide levels within 6-24 hrs, which was not inhibited by cycloheximide(2ug /ml), a protein synthesis inhibitor. In addition, reserpine-induced increase of enkephalin was not inhinited by dactinomycin(5ug / ml), which was found to decrease preproenkephalin mRNA. When preproenkephalin mRNA was measured by Northern blot hybridization with cDNA probe, reserpine elicited a slightly increased preproenkephalin mRNA in PCl2 cells as well as in the rat adrenal glands.
From the present experimental data it is thought that reserpine-induced enhancement of cellular enkephalin content in cultured PCl2 cells may be mediated through the post translational processing along with a slightly increased transcription.
|
|
|
KEYWORD
|
|
|
PC12 Cells, Preproenkephalin, Reserpine
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|